7DW9
Cryo-EM structure of human V2 vasopressin receptor in complex with an Gs protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Vasopressin V2 receptor | polymer | 645 | 71063.7 | 1 | UniProt (P30518) Pfam (PF00001) | Homo sapiens (Human) | V2R,AVPR V2,Antidiuretic hormone receptor,Renal-type arginine vasopressin receptor |
| 2 | B (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 361 | 41879.5 | 1 | Pfam (PF00503) UniProt (by SIFTS) (P63092) UniProt (by SIFTS) (P63096) | Homo sapiens | |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 371 | 40227.0 | 1 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Rat) | Transducin beta chain 1 |
| 4 | D (Y) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G gamma-I |
| 5 | E (N) | Nanobody35 | polymer | 140 | 15343.0 | 1 | synthetic construct | ||
| 6 | F (S) | scFv16 | polymer | 247 | 26277.3 | 1 | synthetic construct | ||
| 7 | G (C) | CYS-TYR-PHE-GLN-ASN-CYS-PRO-ARG-GLY-NH2 | polymer | 10 | 1086.2 | 1 | Pfam (PF00220) UniProt (by SIFTS) (P01185) | Homo sapiens | |
| 8 | H, I, J (R) | CHOLESTEROL | non-polymer | 386.7 | 3 | Chemie (CLR) |
Sequence modifications
R: 1 - 371 (UniProt: P30518)
B: 2 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| His -115 | - | expression tag |
| His -114 | - | expression tag |
| His -113 | - | expression tag |
| His -112 | - | expression tag |
| His -111 | - | expression tag |
| His -110 | - | expression tag |
| His -109 | - | expression tag |
| His -108 | - | expression tag |
| His -107 | - | expression tag |
| His -106 | - | expression tag |
| Ala -105 | - | expression tag |
| Asp -104 | - | expression tag |
| Leu -103 | - | expression tag |
| Glu -102 | - | expression tag |
| Asp -101 | - | expression tag |
| Asn -100 | - | expression tag |
| Trp -99 | - | expression tag |
| Glu -98 | - | expression tag |
| Thr -97 | - | expression tag |
| Leu -96 | - | expression tag |
| Asn -95 | - | expression tag |
| Asp -94 | - | expression tag |
| Asn -93 | - | expression tag |
| Leu -92 | - | expression tag |
| Lys -91 | - | expression tag |
| Val -90 | - | expression tag |
| Ile -89 | - | expression tag |
| Glu -88 | - | expression tag |
| Lys -87 | - | expression tag |
| Ala -86 | - | expression tag |
| Asp -85 | - | expression tag |
| Asn -84 | - | expression tag |
| Ala -83 | - | expression tag |
| Ala -82 | - | expression tag |
| Gln -81 | - | expression tag |
| Val -80 | - | expression tag |
| Lys -79 | - | expression tag |
| Asp -78 | - | expression tag |
| Ala -77 | - | expression tag |
| Leu -76 | - | expression tag |
| Thr -75 | - | expression tag |
| Lys -74 | - | expression tag |
| Met -73 | - | expression tag |
| Arg -72 | - | expression tag |
| Ala -71 | - | expression tag |
| Ala -70 | - | expression tag |
| Ala -69 | - | expression tag |
| Leu -68 | - | expression tag |
| Asp -67 | - | expression tag |
| Ala -66 | - | expression tag |
| Gln -65 | - | expression tag |
| Lys -64 | - | expression tag |
| Ala -63 | - | expression tag |
| Thr -62 | - | expression tag |
| Pro -61 | - | expression tag |
| Pro -60 | - | expression tag |
| Lys -59 | - | expression tag |
| Leu -58 | - | expression tag |
| Glu -57 | - | expression tag |
| Asp -56 | - | expression tag |
| Lys -55 | - | expression tag |
| Ser -54 | - | expression tag |
| Pro -53 | - | expression tag |
| Asp -52 | - | expression tag |
| Ser -51 | - | expression tag |
| Pro -50 | - | expression tag |
| Glu -49 | - | expression tag |
| Met -48 | - | expression tag |
| Lys -47 | - | expression tag |
| Asp -46 | - | expression tag |
| Phe -45 | - | expression tag |
| Arg -44 | - | expression tag |
| His -43 | - | expression tag |
| Gly -42 | - | expression tag |
| Phe -41 | - | expression tag |
| Asp -40 | - | expression tag |
| Ile -39 | - | expression tag |
| Leu -38 | - | expression tag |
| Val -37 | - | expression tag |
| Gly -36 | - | expression tag |
| Gln -35 | - | expression tag |
| Ile -34 | - | expression tag |
| Asp -33 | - | expression tag |
| Asp -32 | - | expression tag |
| Ala -31 | - | expression tag |
| Leu -30 | - | expression tag |
| Lys -29 | - | expression tag |
| Leu -28 | - | expression tag |
| Ala -27 | - | expression tag |
| Asn -26 | - | expression tag |
| Glu -25 | - | expression tag |
| Gly -24 | - | expression tag |
| Lys -23 | - | expression tag |
| Val -22 | - | expression tag |
| Lys -21 | - | expression tag |
| Glu -20 | - | expression tag |
| Ala -19 | - | expression tag |
| Gln -18 | - | expression tag |
| Ala -17 | - | expression tag |
| Ala -16 | - | expression tag |
| Ala -15 | - | expression tag |
| Glu -14 | - | expression tag |
| Gln -13 | - | expression tag |
| Leu -12 | - | expression tag |
| Lys -11 | - | expression tag |
| Thr -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Arg -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Ile -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Tyr -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Val 372 | - | expression tag |
| Phe 373 | - | expression tag |
| Thr 374 | - | expression tag |
| Leu 375 | - | expression tag |
| Glu 376 | - | expression tag |
| Asp 377 | - | expression tag |
| Phe 378 | - | expression tag |
| Val 379 | - | expression tag |
| Gly 380 | - | expression tag |
| Asp 381 | - | expression tag |
| Trp 382 | - | expression tag |
| Glu 383 | - | expression tag |
| Gln 384 | - | expression tag |
| Thr 385 | - | expression tag |
| Ala 386 | - | expression tag |
| Ala 387 | - | expression tag |
| Tyr 388 | - | expression tag |
| Asn 389 | - | expression tag |
| Leu 390 | - | expression tag |
| Asp 391 | - | expression tag |
| Gln 392 | - | expression tag |
| Val 393 | - | expression tag |
| Leu 394 | - | expression tag |
| Glu 395 | - | expression tag |
| Gln 396 | - | expression tag |
| Gly 397 | - | expression tag |
| Gly 398 | - | expression tag |
| Val 399 | - | expression tag |
| Ser 400 | - | expression tag |
| Ser 401 | - | expression tag |
| Leu 402 | - | expression tag |
| Leu 403 | - | expression tag |
| Gln 404 | - | expression tag |
| Asn 405 | - | expression tag |
| Leu 406 | - | expression tag |
| Ala 407 | - | expression tag |
| Val 408 | - | expression tag |
| Ser 409 | - | expression tag |
| Val 410 | - | expression tag |
| Thr 411 | - | expression tag |
| Pro 412 | - | expression tag |
| Ile 413 | - | expression tag |
| Gln 414 | - | expression tag |
| Arg 415 | - | expression tag |
| Ile 416 | - | expression tag |
| Val 417 | - | expression tag |
| Arg 418 | - | expression tag |
| Ser 419 | - | expression tag |
| Gly 420 | - | expression tag |
| Glu 421 | - | expression tag |
| Asn 422 | - | expression tag |
| Ala 423 | - | expression tag |
| Leu 424 | - | expression tag |
| Lys 425 | - | expression tag |
| Ile 426 | - | expression tag |
| Asp 427 | - | expression tag |
| Ile 428 | - | expression tag |
| His 429 | - | expression tag |
| Val 430 | - | expression tag |
| Ile 431 | - | expression tag |
| Ile 432 | - | expression tag |
| Pro 433 | - | expression tag |
| Tyr 434 | - | expression tag |
| Glu 435 | - | expression tag |
| Gly 436 | - | expression tag |
| Leu 437 | - | expression tag |
| Ser 438 | - | expression tag |
| Ala 439 | - | expression tag |
| Asp 440 | - | expression tag |
| Gln 441 | - | expression tag |
| Met 442 | - | expression tag |
| Ala 443 | - | expression tag |
| Gln 444 | - | expression tag |
| Ile 445 | - | expression tag |
| Glu 446 | - | expression tag |
| Glu 447 | - | expression tag |
| Val 448 | - | expression tag |
| Phe 449 | - | expression tag |
| Lys 450 | - | expression tag |
| Val 451 | - | expression tag |
| Val 452 | - | expression tag |
| Tyr 453 | - | expression tag |
| Pro 454 | - | expression tag |
| Val 455 | - | expression tag |
| Asp 456 | - | expression tag |
| Asp 457 | - | expression tag |
| His 458 | - | expression tag |
| His 459 | - | expression tag |
| Phe 460 | - | expression tag |
| Lys 461 | - | expression tag |
| Val 462 | - | expression tag |
| Ile 463 | - | expression tag |
| Leu 464 | - | expression tag |
| Pro 465 | - | expression tag |
| Tyr 466 | - | expression tag |
| Gly 467 | - | expression tag |
| Thr 468 | - | expression tag |
| Leu 469 | - | expression tag |
| Val 470 | - | expression tag |
| Ile 471 | - | expression tag |
| Asp 472 | - | expression tag |
| Gly 473 | - | expression tag |
| Val 474 | - | expression tag |
| Thr 475 | - | expression tag |
| Pro 476 | - | expression tag |
| Asn 477 | - | expression tag |
| Met 478 | - | expression tag |
| Leu 479 | - | expression tag |
| Asn 480 | - | expression tag |
| Tyr 481 | - | expression tag |
| Phe 482 | - | expression tag |
| Gly 483 | - | expression tag |
| Arg 484 | - | expression tag |
| Pro 485 | - | expression tag |
| Tyr 486 | - | expression tag |
| Glu 487 | - | expression tag |
| Gly 488 | - | expression tag |
| Ile 489 | - | expression tag |
| Ala 490 | - | expression tag |
| Val 491 | - | expression tag |
| Phe 492 | - | expression tag |
| Asp 493 | - | expression tag |
| Gly 494 | - | expression tag |
| Lys 495 | - | expression tag |
| Lys 496 | - | expression tag |
| Ile 497 | - | expression tag |
| Thr 498 | - | expression tag |
| Val 499 | - | expression tag |
| Thr 500 | - | expression tag |
| Gly 501 | - | expression tag |
| Thr 502 | - | expression tag |
| Leu 503 | - | expression tag |
| Trp 504 | - | expression tag |
| Asn 505 | - | expression tag |
| Gly 506 | - | expression tag |
| Asn 507 | - | expression tag |
| Lys 508 | - | expression tag |
| Ile 509 | - | expression tag |
| Ile 510 | - | expression tag |
| Asp 511 | - | expression tag |
| Glu 512 | - | expression tag |
| Arg 513 | - | expression tag |
| Leu 514 | - | expression tag |
| Ile 515 | - | expression tag |
| Thr 516 | - | expression tag |
| Pro 517 | - | expression tag |
| Asp 518 | - | expression tag |
| Gly 519 | - | expression tag |
| Ser 520 | - | expression tag |
| Met 521 | - | expression tag |
| Leu 522 | - | expression tag |
| Phe 523 | - | expression tag |
| Arg 524 | - | expression tag |
| Val 525 | - | expression tag |
| Thr 526 | - | expression tag |
| Ile 527 | - | expression tag |
| Asn 528 | - | expression tag |
| Ser 529 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Gly 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Ser 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Gly 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Gly 352 | - | expression tag |
| Ser 353 | - | expression tag |
| Ser 354 | - | expression tag |
| Gly 355 | - | expression tag |
| Val 356 | - | expression tag |
| Ser 357 | - | expression tag |
| Gly 358 | - | expression tag |
| Trp 359 | - | expression tag |
| Arg 360 | - | expression tag |
| Leu 361 | - | expression tag |
| Phe 362 | - | expression tag |
| Lys 363 | - | expression tag |
| Lys 364 | - | expression tag |
| Ile 365 | - | expression tag |
| Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 203737.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1160.0 | |
| All* | Total formula weight | 204897.8 |
