7DW8

Structure of a novel beta-mannanase BaMan113A with mannobiose, N236Y mutation.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Endo-beta-1,4-mannanase	polymer	348	40638.8	2	UniProt (A0A140EH91) Pfam (PF22612)	Bacillus sp. N16-5	BaMan113B
2	C, D (G, H)	beta-D-mannopyranose-(1-4)-beta-D-mannopyranose	branched		342.3	2	In PDB BIRD (PRD_900115) GlyTouCan (G78317QO)
3	E, F (A, B)	water	water		18.0	677	Chemie (HOH)

Sequence modifications

A, B: 1 - 314 (UniProt: A0A140EH91)

PDB	External Database	Details
Met -33	-	initiating methionine
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 142	Glu 142	engineered mutation
Tyr 236	Asn 236	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81277.5
Branched	Number of molecules	2
	Total formula weight	684.6
All*	Total formula weight	81962.1

*Water molecules are not included.