7DVZ

Structure of a novel beta-mannanase BaMan113A from Bacillus sp. N16-5, N236Y mutation.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Endo-beta-1,4-mannanase	polymer	348	40638.8	2	UniProt (A0A140EH91) Pfam (PF22612) In PDB	Bacillus sp. N16-5	BaMan113B
2		water	water		18.0	618	Chemie (HOH)

Sequence modifications

A, B: 1 - 314 (UniProt: A0A140EH91)

PDB	External Database	Details
Met -33	-	initiating methionine
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 142	Glu 142	engineered mutation
Tyr 236	Asn 236	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81277.5
All*	Total formula weight	81277.5

*Water molecules are not included.