7DS7

The Crystal Structure of Leaf-branch compost cutinase from Biortus.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Leaf-branch compost cutinase	polymer	277	29805.3	1	UniProt (G9BY57) Pfam (PF12695) In PDB	Unknown prokaryotic organism	LCC,PET-digesting enzyme,Poly(ethylene terephthalate) hydrolase,PETase
2	A	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	A	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
5		water	water		18.0	114	Chemie (HOH)

Sequence modifications

A: 36 - 293 (UniProt: G9BY57)

PDB	External Database	Details
Met 17	-	initiating methionine
Gly 18	-	expression tag
Ser 19	-	expression tag
Ser 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
Glu 27	-	expression tag
Asn 28	-	expression tag
Leu 29	-	expression tag
Tyr 30	-	expression tag
Phe 31	-	expression tag
Gln 32	-	expression tag
Gly 33	-	expression tag
Gly 34	-	expression tag
Gly 35	-	expression tag
Gly 127	Tyr 127	engineered mutation
Ala 165	Ser 165	engineered mutation
Cys 238	Asp 238	engineered mutation
Ile 243	Phe 243	engineered mutation
Cys 283	Ser 283	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29805.3
Non-Polymers*	Number of molecules	3
	Total formula weight	353.3
All*	Total formula weight	30158.6

*Water molecules are not included.