7DL0

The mutant E310G/A314Y of 3,5-DAHDHcca complex with NADPH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	3,5-diaminohexanoate dehydrogenase	polymer	358	39178.0	2	UniProt (B0VJ11) Pfam (PF00107)	Cloacimonas acidaminovorans (strain Evry)	3,5-DAHDHcca
2	C, D (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
3	E, J, K (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
4	F, M, N (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	G, H (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	I, O (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	L (B)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	1	Chemie (NAP)
8	P (B)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
9	Q, R (A, B)	water	water		18.0	206	Chemie (HOH)

Sequence modifications

A, B: 1 - 352 (UniProt: B0VJ11)

PDB	External Database	Details
Gly 310	Glu 310	engineered mutation
Tyr 314	Ala 314	engineered mutation
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78356.1
Non-Polymers*	Number of molecules	14
	Total formula weight	1831.3
All*	Total formula weight	80187.4

*Water molecules are not included.