7D89

Crystal structure of an inactivated double mutant (E182AE280A) of a novel thermostable GH10 xylanase XynA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta-xylanase	polymer	428	49806.9	1	UniProt (A0A4P8ESF9) Pfam (PF00331) In PDB	Bacillus sp.
2	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3		water	water		18.0	2	Chemie (HOH)

Sequence modifications

A: 1 - 408 (UniProt: A0A4P8ESF9)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 182	Glu 182	engineered mutation
Ala 280	Glu 280	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	49806.9
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	49887.1

*Water molecules are not included.