7D7M
Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Prostaglandin E2 receptor EP4 subtype,Prostaglandin E2 receptor EP4 subtype | polymer | 333 | 36953.5 | 1 | UniProt (P35408) Pfam (PF00001) | Homo sapiens (Human) | PGE2 receptor EP4 subtype,Prostanoid EP4 receptor,PGE2 receptor EP4 subtype,Prostanoid EP4 receptor |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37728.2 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 4 | D (D) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 249 | 29014.8 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein |
| 5 | E (E) | nanobody Nb35 | polymer | 134 | 14680.3 | 1 | Lama glama | ||
| 6 | F (A) | (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid | non-polymer | 352.5 | 1 | Chemie (P2E) |
Sequence modifications
A: 4 - 217 (UniProt: P35408)
A: 260 - 366 (UniProt: P35408)
B: 2 - 340 (UniProt: P62873)
D: 5 - 64 (UniProt: P63092)
D: 204 - 254 (UniProt: P63092)
D: 265 - 394 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Thr 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Gln 7 | Asn 7 | engineered mutation |
| Gln 177 | Asn 177 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Leu 367 | - | expression tag |
| Glu 368 | - | expression tag |
| Leu 369 | - | expression tag |
| Glu 370 | - | expression tag |
| Val 371 | - | expression tag |
| Leu 372 | - | expression tag |
| Phe 373 | - | expression tag |
| Gln 374 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp 49 | Gly 49 | engineered mutation |
| Asn 50 | Glu 50 | engineered mutation |
| Tyr 63 | Leu 63 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 196 | - | linker |
| Gly 197 | - | linker |
| Ser 198 | - | linker |
| Gly 199 | - | linker |
| Gly 200 | - | linker |
| Ser 201 | - | linker |
| Gly 202 | - | linker |
| Gly 203 | - | linker |
| Asp 249 | Ala 249 | engineered mutation |
| Asp 252 | Ser 252 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 372 | Ile 372 | engineered mutation |
| Ile 375 | Val 375 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 126237.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 352.5 | |
| All* | Total formula weight | 126590.3 |
