7D7D

CryoEM structure of gp45-dependent transcription activation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (U9ZUN7)	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (A0A080FHH4)	Escherichia coli 1-392-07_S4_C3	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (D7Y6A2)	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (T)	DNA (template strand)	polymer	59	18203.7	1		Escherichia virus T4
5	F (F)	gp55	polymer	185	21565.2	1		Escherichia virus T4
6	G (N)	DNA (nontemplate strand)	polymer	59	18155.7	1		Escherichia virus T4
7	H (K)	RNA polymerase-associated protein Gp33	polymer	132	15014.9	1	UniProt (P13338) Pfam (PF16805)	Enterobacteria phage T4
8	I (E)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (A0A070UPX4)	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
9	J, K, L (G, H, I)	DNA polymerase clamp	polymer	236	25952.4	3	UniProt (P04525) Pfam (PF02916) Pfam (PF09116)	Enterobacteria phage T4	DNA polymerase accessory protein 45,DNA polymerase sliding clamp,Gp45
10	M (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
11	N, O (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

K: 1 - 112 (UniProt: P13338)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

G, H, I: 1 - 228 (UniProt: P04525)

PDB	External Database	Details
Leu 229	-	expression tag
Glu 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	540351.2
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	540506.3

*Water molecules are not included.