7D77

Cryo-EM structure of the cortisol-bound adhesion receptor GPR97-Go complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	226	25286.9	1	Pfam (PF00503) UniProt (by SIFTS) (P09471) In PDB	Homo sapiens
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38245.8	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	R	Adhesion G protein-coupled receptor G3; GPR97	polymer	561	62767.1	1	Pfam (PF01825) Pfam (PF00002) UniProt (by SIFTS) (Q86Y34) In PDB	Homo sapiens
5	S	scFv16	polymer	250	26610.6	1		synthetic construct
6	A, R	PALMITIC ACID	non-polymer		256.4	3	Chemie (PLM)
7	R	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01)
8	R	(11alpha,14beta)-11,17,21-trihydroxypregn-4-ene-3,20-dione	non-polymer		362.5	1	Chemie (HCY)
9	R	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
10		water	water		18.0	1	Chemie (HOH)

Sequence modifications

B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	160771.6
Non-Polymers*	Number of molecules	7
	Total formula weight	2391.8
All*	Total formula weight	163163.3

*Water molecules are not included.