7D69

Cryo-EM structure of the nucleosome containing Giardia histones

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, E)	Histone H3	polymer	149	16639.6	2	UniProt (V6TEE9) Pfam (PF00125) UniProt (by SIFTS) (E2RU29)	Giardia intestinalis
2	B, E (B, F)	Histone H4	polymer	102	11407.2	2	UniProt (E2RU60) Pfam (PF15511) UniProt (by SIFTS) (A8BUJ9)	Giardia intestinalis
3	C, F (D, H)	Histone H2B	polymer	133	14903.1	2	UniProt (V6TJV6) Pfam (PF00125) UniProt (by SIFTS) (A8BI78)	Giardia intestinalis
4	G, H (C, G)	Histone H2A	polymer	127	14188.2	2	UniProt (E2RU15) Pfam (PF00125) UniProt (by SIFTS) (A8BHS7)	Giardia intestinalis
5	I (I)	601L DNA (145-MER)	polymer	145	44761.5	1		synthetic construct
6	J (J)	601L DNA (145-MER)	polymer	145	44752.5	1		synthetic construct

Sequence modifications

A, E: 0 - 145 (UniProt: V6TEE9)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

B, F: 0 - 98 (UniProt: E2RU60)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 129 (UniProt: V6TJV6)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

C, G: 0 - 123 (UniProt: E2RU15)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	203790.2
All*	Total formula weight	203790.2

*Water molecules are not included.