7D3S

Human SECR in complex with an engineered Gs heterotrimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	P	Secretin	polymer	27	3045.4	1	UniProt (P09683) Pfam (PF00123) In PDB	Homo sapiens
2	R	Secretin receptor	polymer	434	49742.4	1	UniProt (P47872) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	SCT-R
3	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44255.7	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
4	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Rat)	Transducin beta chain 1
5	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7547.7	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (Bovine)	G gamma-I
6	N	nanobody Nb35	polymer	137	15015.7	1		unidentified

Sequence modifications

R: 23 - 440 (UniProt: P47872)

PDB	External Database	Details
Asp 13	-	expression tag
Tyr 14	-	expression tag
Lys 15	-	expression tag
Asp 16	-	expression tag
Asp 17	-	expression tag
Asp 18	-	expression tag
Asp 19	-	expression tag
Ala 20	-	expression tag
Met 21	-	expression tag
Gly 22	-	expression tag
Glu 441	-	expression tag
Asn 442	-	expression tag
Leu 443	-	expression tag
Tyr 444	-	expression tag
Phe 445	-	expression tag
Gly 446	-	expression tag

A: 5 - 384 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation
Tyr 63	Leu 63	engineered mutation
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 362	Ile 372	engineered mutation
Ile 365	Val 375	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

G: 1 - 67 (UniProt: P63212)

PDB	External Database	Details
Ser 68	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	158351.3
All*	Total formula weight	158351.3

*Water molecules are not included.