7D06

Cryo EM structure of the nucleotide free Acinetobacter MlaFEDB complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Intermembrane phospholipid transport system permease protein MlaE	polymer	258	27322.4	2	UniProt (V5V9F4) Pfam (PF02405)	Acinetobacter baumannii
2	B, E (B, E)	ABC transporter ATP-binding protein	polymer	273	30070.5	2	UniProt (A0A086HZU3) Pfam (PF00005)	Acinetobacter baumannii	ATP-binding cassette domain-containing protein,Iron(III) ABC transporter,ATP-binding protein,Phospholipid ABC transporter ATP-binding protein MlaF,Toluene tolerance efflux transporter
3	C, F (C, F)	Anti-sigma factor antagonist	polymer	103	11913.8	2	UniProt (V5V9K5) Pfam (PF13466)	Acinetobacter baumannii	Phospholipid transport system transporter-binding protein,STAS domain protein,STAS domain-containing protein,Toluene tolerance protein,Ttg2E
4	G, H, I, J, K... (G, H, I, J, K...)	MCE family protein	polymer	226	24135.3	6	UniProt (V5V921) Pfam (PF02470)	Acinetobacter baumannii	Mammalian cell entry protein,Mce related family protein,Outer membrane lipid asymmetry maintenance protein MlaD,Phospholipid/cholesterol/gamma-HCH transport system substrate-binding protein,Putative phospholipid ABC transporter-binding protein MlaD,Toluene tolerance efflux transporter,Toluene tolerance efflux transporter
5	M, N, O, P, Q... (A, D, I)	(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE	non-polymer		749.0	12	Chemie (PGV)

Sequence modifications

B, E: 2 - 273 (UniProt: A0A086HZU3)

PDB	External Database	Details
Met 1	-	initiating methionine

C, F: 2 - 95 (UniProt: V5V9K5)

PDB	External Database	Details
Val 1	-	expression tag
Leu 96	-	expression tag
Glu 97	-	expression tag
His 98	-	expression tag
His 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	283425.2
Non-Polymers*	Number of molecules	12
	Total formula weight	8988.1
All*	Total formula weight	292413.3

*Water molecules are not included.