7CXN

Architecture of a SARS-CoV-2 mini replication and transcription complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-directed RNA polymerase	polymer	942	108161.5	1	UniProt (P0DTD1) Pfam (PF06478) Pfam (PF00680)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,Pol,RdRp,Non-structural protein 12,nsp12
2	B, D (B, D)	Non-structural protein 8	polymer	198	21903.0	2	UniProt (P0DTD1) Pfam (PF08717)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,nsp8
3	C (C)	Non-structural protein 7	polymer	83	9248.8	1	UniProt (P0DTD1) Pfam (PF08716)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,nsp7
4	E (I)	Primer RNA	polymer	25	8172.9	1		Severe acute respiratory syndrome coronavirus 2
5	F (J)	Template RNA	polymer	58	18462.0	1		Severe acute respiratory syndrome coronavirus 2
6	G (L)	RNA (5'-R(P*UP*UP*UP*UP*UP*U)-3')	polymer	6	1884.2	1		Severe acute respiratory syndrome coronavirus 2
7	H, I (F, E)	Helicase	polymer	601	66930.5	2	UniProt (P0DTD1) Pfam (PF20632) Pfam (PF20633) Pfam (PF20631) Pfam (PF13087)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,Hel,Non-structural protein 13,nsp13
8	J, K, L, M, N... (A, F, E)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)

Sequence modifications

A: 1 - 932 (UniProt: P0DTD1)

PDB	External Database	Details
Asn 910	Asp 5302	engineered mutation
His 933	-	expression tag
His 934	-	expression tag
His 935	-	expression tag
His 936	-	expression tag
His 937	-	expression tag
His 938	-	expression tag
His 939	-	expression tag
His 940	-	expression tag
His 941	-	expression tag
His 942	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	323596.5
Non-Polymers*	Number of molecules	8
	Total formula weight	523.3
All*	Total formula weight	324119.8

*Water molecules are not included.