7CX2
Cryo-EM structure of the PGE2-bound EP2-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Prostaglandin E2 receptor EP2 subtype | polymer | 358 | 39800.4 | 1 | UniProt (P43116) Pfam (PF00001) | Homo sapiens (Human) | PGE2 receptor EP2 subtype,Prostanoid EP2 receptor |
| 2 | B (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39489.2 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (Human) | Transducin beta chain 1 |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 5 | E (N) | Nanobody-35 | polymer | 128 | 13885.4 | 1 | synthetic construct | ||
| 6 | F (R) | (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid | non-polymer | 352.5 | 1 | Chemie (P2E) PubChem (158) PubChem (9691) PubChem (122348) PubChem (3246816) PubChem (3246832) PubChem (5283061) PubChem (5283086) PubChem (5283116) PubChem (5283213) PubChem (5353587) PubChem (6445999) PubChem (6450019) PubChem (9874974) PubChem (9950404) PubChem (10247450) PubChem (11221902) PubChem (11581143) PubChem (12041746) PubChem (12314577) PubChem (12314580) PubChem (12314582) PubChem (12314584) PubChem (12314585) PubChem (12314588) PubChem (12314590) PubChem (12314594) PubChem (16219826) PubChem (71195864) PubChem (35021823) PubChem (35021833) PubChem (35027359) PubChem (40575698) PubChem (44444717) PubChem (176729059) PubChem (177546178) PubChem (26436117) PubChem (49822699) PubChem (40575690) PubChem (91886115) PubChem (5280360) PubChem (97041839) PubChem (53477468) PubChem (54466054) PubChem (54466055) PubChem (54466056) PubChem (54466057) PubChem (57357999) PubChem (12314587) PubChem (156359195) PubChem (59294054) PubChem (171042408) PubChem (59881728) PubChem (59926133) PubChem (59941509) PubChem (59968515) PubChem (67937739) PubChem (70419675) PubChem (71082424) PubChem (71401211) PubChem (73357245) PubChem (90689772) PubChem (90798199) PubChem (91019309) PubChem (92450042) PubChem (92966520) PubChem (97041838) PubChem (122176715) PubChem (122702174) PubChem (123841661) PubChem (124350425) PubChem (124406582) PubChem (124406583) PubChem (124524501) PubChem (131954610) PubChem (139503973) PubChem (149202340) PubChem (154166399) PubChem (155114512) PubChem (156358885) PubChem (156359009) PubChem (156681952) PubChem (156681960) PubChem (162705518) PubChem (163091488) PubChem (163091489) PubChem (166639831) PubChem (166639839) PubChem (166639992) PubChem (169502219) PubChem (171157690) PubChem (172878425) PubChem (173974797) PubChem (175594575) | |||
| 7 | G (R) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 146719.6 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 352.5 | |
| All* | Total formula weight | 147072.1 |
