7CTV

Crystal structure of Arabidopsis thaliana SOBIR1 kinase domain D489A mutant in complex with AMP-PNP and magnesium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1	polymer	336	37784.5	2	UniProt (Q9SKB2) Pfam (PF00069)	Arabidopsis thaliana (Mouse-ear cress)	Protein EVERSHED,Protein SUPPRESSOR OF BIR1-1
2	C, E (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	D, F (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)

Sequence modifications

A, B: 326 - 640 (UniProt: Q9SKB2)

PDB	External Database	Details
Met 305	-	initiating methionine
Gly 306	-	expression tag
Ser 307	-	expression tag
Ser 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
Ser 315	-	expression tag
Ser 316	-	expression tag
Gly 317	-	expression tag
Leu 318	-	expression tag
Val 319	-	expression tag
Pro 320	-	expression tag
Arg 321	-	expression tag
Gly 322	-	expression tag
Ser 323	-	expression tag
His 324	-	expression tag
Met 325	-	expression tag
Ala 489	Asp 489	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	75569.0
Non-Polymers*	Number of molecules	4
	Total formula weight	1061.0
All*	Total formula weight	76630.0

*Water molecules are not included.