7CTS
Open form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A inactivation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alpha/beta hydrolase family protein | polymer | 263 | 28889.5 | 1 | UniProt (W0TJ64) | Saccharomonospora viridis | Cutinase |
| 2 | B (A) | 1,4-DIETHYLENE DIOXIDE | non-polymer | 88.1 | 1 | Chemie (DIO) | |||
| 3 | C (A) | BICINE | non-polymer | 163.2 | 1 | Chemie (BCN) | |||
| 4 | D (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 5 | E (A) | water | water | 18.0 | 445 | Chemie (HOH) |
Sequence modifications
A: 47 - 304 (UniProt: W0TJ64)
| PDB | External Database | Details |
|---|---|---|
| Gly 45 | - | expression tag |
| Pro 46 | - | expression tag |
| His 123 | Gln 123 | engineered mutation |
| Ala 138 | Gln 138 | engineered mutation |
| Ala 176 | Ser 176 | engineered mutation |
| His 202 | Asn 202 | engineered mutation |
| Pro 226 | Ser 226 | engineered mutation |
| Ser 228 | Arg 228 | engineered mutation |
| Cys 250 | Asp 250 | engineered mutation |
| Cys 296 | Glu 296 | engineered mutation |
| Lys 305 | - | expression tag |
| Leu 306 | - | expression tag |
| Asn 307 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 28889.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 291.4 | |
| All* | Total formula weight | 29180.9 |
