7CRQ

NSD3 bearing E1181K/T1232A dual mutation in complex with 187-bp NCP (2:1 binding mode)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (M, E)	Histone H3	polymer	135	15223.8	2	UniProt (Q92133) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	122	13524.8	2	UniProt (Q6AZK7) Pfam (PF00125) UniProt (by SIFTS) (P02281)	Xenopus tropicalis (Western clawed frog)
5	I (A)	DNA (168-MER)	polymer	187	57488.6	1		Xenopus laevis
6	J (K)	DNA (168-MER)	polymer	187	57982.9	1		Xenopus laevis
7	K, L (I, L)	Histone-lysine N-methyltransferase NSD3	polymer	758	85454.4	2	UniProt (Q9BZ95) Pfam (PF17907) Pfam (PF00856) Pfam (PF17982) Pfam (PF23011) Pfam (PF22908) Pfam (PF23004) Pfam (PF00855)	Homo sapiens (Human)	Nuclear SET domain-containing protein 3,Protein whistle,WHSC1-like 1 isoform 9 with methyltransferase activity to lysine,Wolf-Hirschhorn syndrome candidate 1-like protein 1,WHSC1-like protein 1
8	M, T (I, L)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
9	N, O, P, Q, R... (I, L)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

M, E: 1 - 135 (UniProt: Q92133)

PDB	External Database	Details
Nle 36	Lys 37	engineered mutation
Nle 90	Met 91	engineered mutation
Nle 120	Met 121	engineered mutation

I, L: 680 - 1437 (UniProt: Q9BZ95)

PDB	External Database	Details
Lys 1181	Glu 1181	engineered mutation
Ala 1232	Thr 1232	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	394360.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1189.3
All*	Total formula weight	395549.5

*Water molecules are not included.