7CRO

NSD2 bearing E1099K/T1150A dual mutation in complex with 187-bp NCP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (M, E)	Histone H3	polymer	135	15223.8	2	UniProt (Q92133) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	122	13524.8	2	UniProt (Q6AZK7) Pfam (PF00125) UniProt (by SIFTS) (P02281)	Xenopus tropicalis (Western clawed frog)
5	I (A)	DNA (168-MER)	polymer	187	57488.6	1		Xenopus laevis
6	J (K)	DNA (168-MER)	polymer	187	57982.9	1		Xenopus laevis
7	K (I)	Histone-lysine N-methyltransferase NSD2	polymer	705	79688.8	1	UniProt (O96028) Pfam (PF00855) Pfam (PF17907) Pfam (PF00856) Pfam (PF23011) Pfam (PF17982) Pfam (PF22908) Pfam (PF23004) Pfam (PF00628)	Homo sapiens (Human)	Multiple myeloma SET domain-containing protein,MMSET,Nuclear SET domain-containing protein 2,Protein trithorax-5,Wolf-Hirschhorn syndrome candidate 1 protein
8	L (I)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
9	M, N, O (I)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

M, E: 1 - 135 (UniProt: Q92133)

PDB	External Database	Details
Nle 36	Lys 37	engineered mutation
Nle 90	Met 91	engineered mutation
Nle 120	Met 121	engineered mutation

I: 661 - 1365 (UniProt: O96028)

PDB	External Database	Details
Lys 1099	Glu 1099	engineered mutation
Ala 1150	Thr 1150	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	303140.3
Non-Polymers*	Number of molecules	4
	Total formula weight	594.7
All*	Total formula weight	303735.0

*Water molecules are not included.