7CM8

High resolution crystal structure of M92A mutant of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cysteine synthase	polymer	350	37144.3	1	UniProt (P45040) Pfam (PF00291)	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (Haemophilus influenzae Rd KW20)	CSase,O-acetylserine (thiol)-lyase,OAS-TL,O-acetylserine sulfhydrylase
2	B, C, E, F, G (A)	SODIUM ION	non-polymer		23.0	5	Chemie (NA)
3	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	H (A)	water	water		18.0	77	Chemie (HOH)

Sequence modifications

A: 1 - 316 (UniProt: P45040)

PDB	External Database	Details
Met -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 92	Met 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37144.3
Non-Polymers*	Number of molecules	6
	Total formula weight	207.0
All*	Total formula weight	37351.3

*Water molecules are not included.