7CKZ

Cryo-EM structure of Dopamine and LY3154207 bound dopamine receptor DRD1-Gs signaling complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45769.6	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	348	38257.8	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (N)	Nanobody 35	polymer	156	16926.1	1		Lama glama
5	E (R)	D(1A) dopamine receptor	polymer	446	49339.2	1	UniProt (P21728) Pfam (PF00001)	Homo sapiens (Human)	Dopamine D1 receptor
6	F (R)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)
7	G (R)	L-DOPAMINE	non-polymer		153.2	1	Chemie (LDP)
8	H (R)	2-[2,6-bis(chloranyl)phenyl]-1-[(1S,3R)-3-(hydroxymethyl)-1-methyl-5-(3-methyl-3-oxidanyl-butyl)-3,4-dihydro-1H-isoquinolin-2-yl]ethanone	non-polymer		450.4	1	Chemie (G4C)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Thr 205	Ser 205	engineered mutation
Ala 226	Gly 226	engineered mutation
Ser 366	Ala 366	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	158153.7
Non-Polymers*	Number of molecules	3
	Total formula weight	990.2
All*	Total formula weight	159144.0

*Water molecules are not included.