7CKX
Cryo-EM structure of A77636 bound dopamine receptor DRD1-Gs signaling complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45769.6 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39141.8 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 4 | D (N) | Nanobody 35 | polymer | 156 | 16926.1 | 1 | Lama glama | ||
| 5 | E (R) | D(1A) dopamine receptor | polymer | 453 | 50162.9 | 1 | UniProt (P21728) Pfam (PF00001) | Homo sapiens (Human) | Dopamine D1 receptor |
| 6 | F (R) | (1R,3S)-3-(1-adamantyl)-1-(aminomethyl)-3,4-dihydro-1H-isochromene-5,6-diol | non-polymer | 329.4 | 1 | Chemie (G3O) | |||
| 7 | G (R) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
R: 1 - 446 (UniProt: P21728)
| PDB | External Database | Details |
|---|---|---|
| Thr 205 | Ser 205 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 159861.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 716.1 | |
| All* | Total formula weight | 160577.6 |
