7CGS

Crystal endo-deglycosylated hydroxynitrile lyase isozyme 5 mutant L343F from Prunus communis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	(R)-mandelonitrile lyase	polymer	542	59220.6	1	UniProt (A0A5E4GBK6) Pfam (PF00732) Pfam (PF05199) UniProt (by SIFTS) (O24243) In PDB	Prunus dulcis (Almond)	deglycosylated hydroxynitrile lyase isozyme 5
2	A	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG)
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5	A	BICINE	non-polymer		163.2	1	Chemie (BCN)
6		water	water		18.0	725	Chemie (HOH)

Sequence modifications

A: 1 - 532 (UniProt: A0A5E4GBK6)

PDB	External Database	Details
Glu -9	-	expression tag
Ala -8	-	expression tag
Glu -7	-	expression tag
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Phe 343	Leu 370	engineered mutation
Met 532	Gln 559	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	59220.6
Non-Polymers*	Number of molecules	11
	Total formula weight	2840.5
All*	Total formula weight	62061.1

*Water molecules are not included.