7CEC

Structure of alpha6beta1 integrin in complex with laminin-511

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Integrin alpha-6	polymer	627	69776.0	1	UniProt (P23229) Pfam (PF01839) Pfam (PF08441) In PDB	Homo sapiens (Human)	CD49 antigen-like family member F,VLA-6
2	B	Integrin beta-1	polymer	454	50510.9	1	UniProt (P05556) Pfam (PF17205) Pfam (PF00362) In PDB	Homo sapiens (Human)	Fibronectin receptor subunit beta,Glycoprotein IIa,GPIIA,VLA-4 subunit beta
3	C	Laminin subunit alpha-5	polymer	674	73414.2	1	UniProt (O15230) Pfam (PF06009) Pfam (PF00054) Pfam (PF02210) In PDB	Homo sapiens (Human)	Laminin-10 subunit alpha,Laminin-11 subunit alpha,Laminin-15 subunit alpha
4	D	Laminin subunit beta-1	polymer	74	8552.8	1	UniProt (P07942) In PDB	Homo sapiens (Human)	Laminin B1 chain,Laminin-1 subunit beta,Laminin-10 subunit beta,Laminin-12 subunit beta,Laminin-2 subunit beta,Laminin-6 subunit beta,Laminin-8 subunit beta
5	E	Laminin subunit gamma-1	polymer	83	9483.7	1	UniProt (P11047) In PDB	Homo sapiens (Human)	Laminin B2 chain,Laminin-1 subunit gamma,Laminin-10 subunit gamma,Laminin-11 subunit gamma,Laminin-2 subunit gamma,Laminin-3 subunit gamma,Laminin-4 subunit gamma,Laminin-6 subunit gamma,Laminin-7 subunit gamma,Laminin-8 subunit gamma,Laminin-9 subunit gamma,S-laminin subunit gamma,S-LAM gamma
6	F	TS2/16 VH(S112C)-SARAH,TS2/16 VH(S112C)-SARAH	polymer	172	19464.0	1	Pfam (PF11629) UniProt (by SIFTS) (Q13043) In PDB	Mus musculus (Mouse)
7	G	TS2/16 VL-SARAH(S37C),TS2/16 VL-SARAH(S37C)	polymer	164	18270.7	1	Pfam (PF11629) UniProt (by SIFTS) (Q13043) In PDB	Mus musculus (Mouse)
8	H	HUTS-4 VH(S112C)-SARAH,HUTS-4 VH(S112C)-SARAH	polymer	174	19760.2	1	Pfam (PF11629) UniProt (by SIFTS) (Q13043) In PDB	Mus musculus (Mouse)
9	I	HUTS-4 VL(C87Y)-SARAH(S37C),HUTS-4 VL(C87Y)-SARAH(S37C)	polymer	159	17701.0	1	Pfam (PF11629) UniProt (by SIFTS) (Q13043) In PDB	Mus musculus (Mouse)
10	J	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
11	A, C	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
12	A, B, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
13	B	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)

Sequence modifications

A: 1 - 618 (UniProt: P23229)

PDB	External Database	Details
Gly 619	-	expression tag
Ser 620	-	expression tag
Leu 621	-	expression tag
Glu 622	-	expression tag
Asn 623	-	expression tag
Leu 624	-	expression tag
Tyr 625	-	expression tag
Phe 626	-	expression tag
Gln 627	-	expression tag

B: 1 - 445 (UniProt: P05556)

PDB	External Database	Details
Gly 446	-	expression tag
Gly 447	-	expression tag
Leu 448	-	expression tag
Glu 449	-	expression tag
Asn 450	-	expression tag
Leu 451	-	expression tag
Tyr 452	-	expression tag
Phe 453	-	expression tag
Gln 454	-	expression tag

C: 2655 - 3327 (UniProt: O15230)

PDB	External Database	Details
Gly 2654	-	expression tag
Cys 2723	Ile 2723	engineered mutation

D: 1714 - 1786 (UniProt: P07942)

PDB	External Database	Details
Gly 1713	-	expression tag

E: 1528 - 1609 (UniProt: P11047)

PDB	External Database	Details
Gly 1527	-	expression tag
Cys 1585	Asp 1585	engineered mutation

F: 0 - 113 (PDB: 7CEC)
F: 114 - 164 (PDB: 7CEC)
G: -3 - 108 (PDB: 7CEC)
G: 109 - 159 (PDB: 7CEC)
H: 0 - 113 (PDB: 7CEC)
H: 114 - 164 (PDB: 7CEC)
I: 0 - 108 (PDB: 7CEC)
I: 109 - 159 (PDB: 7CEC)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	286933.6
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	13
	Total formula weight	1652.4
All*	Total formula weight	289010.4

*Water molecules are not included.