7C97
Cryo-EM structure of an Escherichia coli RNAP-promoter open complex (RPo) with SspA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (H) | DNA (63-mer) | polymer | 63 | 19671.7 | 1 | Escherichia coli | ||
| 2 | B, C (I, J) | Stringent starvation protein A | polymer | 212 | 24332.9 | 2 | UniProt (H4IXP2) Pfam (PF02798) Pfam (PF00043) UniProt (by SIFTS) (P0ACA3) | Escherichia coli | |
| 3 | D, E, F (K, A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 3 | UniProt (F4VJT6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 4 | G (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150804.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli (strain K12) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 5 | H (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (M9GTE2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 6 | I (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (T9C803) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 7 | J (F) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) | Escherichia coli | Sigma-70 |
| 8 | K (G) | DNA (63-mer) | polymer | 63 | 19159.3 | 1 | Escherichia coli | ||
| 9 | L (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | M, N (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
C: 1 - 1342 (UniProt: P0A8V2)
| PDB | External Database | Details |
|---|---|---|
| Val 516 | Asp 516 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 583946.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 584101.4 |
