7C71

Crystal structure of beta-glycosides-binding protein (E117A) of ABC transporter in an open state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Sugar ABC transporter, periplasmic sugar-binding protein	polymer	423	45965.3	2	UniProt (Q53W80) Pfam (PF13416)	Thermus thermophilus HB8
2	C, J, K (A, B)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
3	D, E, L (A, B)	SULFUR DIOXIDE	non-polymer		64.1	3	Chemie (SO2)
4	F (A)	SULFITE ION	non-polymer		80.1	1	Chemie (SO3)
5	G, H (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	I, M, N (A, B)	TRIETHYLENE GLYCOL	non-polymer		150.2	3	Chemie (PGE)
7	O, P (A, B)	water	water		18.0	611	Chemie (HOH)

Sequence modifications

A, B: 1 - 416 (UniProt: Q53W80)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 117	Glu 135	engineered mutation
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	91930.5
Non-Polymers*	Number of molecules	12
	Total formula weight	953.3
All*	Total formula weight	92883.8

*Water molecules are not included.