7C6V
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to laminaritriose (Form II)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Sugar ABC transporter, periplasmic sugar-binding protein | polymer | 422 | 45906.2 | 3 | UniProt (Q53W80) Pfam (PF13416) | Thermus thermophilus HB8 | |
| 2 | D, E, F (D, E, F) | beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose | branched | 504.4 | 3 | In PDB GlyTouCan (G00024MO) | |||
| 3 | G, L, P (A, B, C) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 4 | H, I, M, R, S (A, B, C) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 5 | J, K, N (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 6 | O (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 7 | Q (C) | SULFITE ION | non-polymer | 80.1 | 1 | Chemie (SO3) | |||
| 8 | T, U, V (A, B, C) | water | water | 18.0 | 52 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 415 (UniProt: Q53W80)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Arg 174 | Lys 192 | engineered mutation |
| Thr 175 | Asn 193 | engineered mutation |
| Pro 176 | Ser 194 | engineered mutation |
| - | Trp 195 | deletion |
| Arg 177 | Asp 196 | engineered mutation |
| Thr 178 | Val 197 | engineered mutation |
| His 416 | - | expression tag |
| His 417 | - | expression tag |
| His 418 | - | expression tag |
| His 419 | - | expression tag |
| His 420 | - | expression tag |
| His 421 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 137718.6 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1513.3 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 959.1 | |
| All* | Total formula weight | 140191.0 |
