7C6M

Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to cellotetraose (Form I)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sugar ABC transporter, periplasmic sugar-binding protein	polymer	422	45906.2	1	UniProt (Q53W80) Pfam (PF13416) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
2	B	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		666.6	1	In PDB BIRD (PRD_900011) GlyTouCan (G00025MO)		beta-cellotetraose
3	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	A	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	240	Chemie (HOH)

Sequence modifications

A: 1 - 415 (UniProt: Q53W80)

PDB	External Database	Details
Met 0	-	initiating methionine
Arg 174	Lys 192	engineered mutation
Thr 175	Asn 193	engineered mutation
Pro 176	Ser 194	engineered mutation
-	Trp 195	deletion
Arg 177	Asp 196	engineered mutation
Thr 178	Val 197	engineered mutation
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45906.2
Branched	Number of molecules	1
	Total formula weight	666.6
Non-Polymers*	Number of molecules	4
	Total formula weight	281.2
All*	Total formula weight	46854.0

*Water molecules are not included.