7C6K
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to cellotriose (Form I)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Sugar ABC transporter, periplasmic sugar-binding protein | polymer | 422 | 45906.2 | 1 | UniProt (Q53W80) Pfam (PF13416) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 2 | B (B) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 504.4 | 1 | In PDB BIRD (PRD_900021) GlyTouCan (G14338VK) | beta-cellotriose | ||
| 3 | C, D, E (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 4 | F (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 5 | G (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 6 | H (A) | PYROPHOSPHATE 2- | non-polymer | 176.0 | 1 | Chemie (POP) | |||
| 7 | I (A) | water | water | 18.0 | 378 | Chemie (HOH) |
Sequence modifications
A: 1 - 415 (UniProt: Q53W80)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Arg 174 | Lys 192 | engineered mutation |
| Thr 175 | Asn 193 | engineered mutation |
| Pro 176 | Ser 194 | engineered mutation |
| - | Trp 195 | deletion |
| Arg 177 | Asp 196 | engineered mutation |
| Thr 178 | Val 197 | engineered mutation |
| His 416 | - | expression tag |
| His 417 | - | expression tag |
| His 418 | - | expression tag |
| His 419 | - | expression tag |
| His 420 | - | expression tag |
| His 421 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 45906.2 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 504.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 440.4 | |
| All* | Total formula weight | 46851.1 |
