7C6F

Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in an open state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Sugar ABC transporter, periplasmic sugar-binding protein	polymer	422	45906.2	1	UniProt (Q53W80) Pfam (PF13416)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
2	B, C, D, E (A)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
3	F, G (A)	SULFITE ION	non-polymer		80.1	2	Chemie (SO3)
4	H (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
5	I, J (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	K (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
7	L (A)	water	water		18.0	401	Chemie (HOH)

Sequence modifications

A: 1 - 415 (UniProt: Q53W80)

PDB	External Database	Details
Met 0	-	initiating methionine
Arg 174	Lys 192	engineered mutation
Thr 175	Asn 193	engineered mutation
Pro 176	Ser 194	engineered mutation
-	Trp 195	deletion
Arg 177	Asp 196	engineered mutation
Thr 178	Val 197	engineered mutation
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45906.2
Non-Polymers*	Number of molecules	10
	Total formula weight	635.3
All*	Total formula weight	46541.5

*Water molecules are not included.