7C5M

Crystal Structure of C150A+H177A mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with G3P at 1.8 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (O, P, Q, R)	Glyceraldehyde-3-phosphate dehydrogenase	polymer	352	37788.8	4	UniProt (A0A140NCK4)	Escherichia coli BL21(DE3)
2	E, I, L, P (O, P, Q, R)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
3	F (O)	3-PHOSPHOGLYCERIC ACID	non-polymer		186.1	1	Chemie (3PG)
4	G, H (O)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	J, M, Q (P, Q, R)	GLYCERALDEHYDE-3-PHOSPHATE	non-polymer		170.1	3	Chemie (G3H)
6	K, N, O, R (P, Q, R)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
7	S, T, U, V (O, P, Q, R)	water	water		18.0	1474	Chemie (HOH)

Sequence modifications

O, P, Q, R: 1 - 333 (UniProt: A0A140NCK4)

PDB	External Database	Details
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 150	Cys 150	engineered mutation
Ala 177	His 177	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151155.2
Non-Polymers*	Number of molecules	14
	Total formula weight	3788.1
All*	Total formula weight	154943.3

*Water molecules are not included.