7C5L
Crystal Structure of C150S mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli at 2.1 Angstrom resolution
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | O, P, Q, R | Glyceraldehyde-3-phosphate dehydrogenase | polymer | 352 | 37871.9 | 4 | UniProt (A0A140NCK4) Pfam (PF00044) Pfam (PF02800) In PDB | Escherichia coli BL21(DE3) | |
2 | O, P, Q, R | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 4 | Chemie (NAD) | |||
3 | O, P, Q, R | CHLORIDE ION | non-polymer | 35.5 | 5 | Chemie (CL) | |||
4 | O | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
5 | P | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
6 | water | water | 18.0 | 988 | Chemie (HOH) |
Sequence modifications
O, P, Q, R: 1 - 333 (UniProt: A0A140NCK4)
PDB | External Database | Details |
---|---|---|
His -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
Ser -12 | - | expression tag |
Ser -11 | - | expression tag |
Gly -10 | - | expression tag |
Leu -9 | - | expression tag |
Val -8 | - | expression tag |
Pro -7 | - | expression tag |
Arg -6 | - | expression tag |
Gly -5 | - | expression tag |
Ser -4 | - | expression tag |
His -3 | - | expression tag |
Met -2 | - | expression tag |
Ala -1 | - | expression tag |
Ser 0 | - | expression tag |
Ser 150 | Cys 150 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 151487.4 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 2988.0 | |
All* | Total formula weight | 154475.4 |