7C25

Glycosidase Wild Type at pH8.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Isomaltose glucohydrolase	polymer	405	44044.1	1	UniProt (D2PPM8) Pfam (PF00723)	Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399)
2	B, C, D, E (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
3	F (A)	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
4	G (A)	AMMONIUM ION	non-polymer		18.0	1	Chemie (NH4)
5	H (A)	water	water		18.0	415	Chemie (HOH)

Sequence modifications

A: 1 - 385 (UniProt: D2PPM8)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44044.1
Non-Polymers*	Number of molecules	6
	Total formula weight	578.5
All*	Total formula weight	44622.6

*Water molecules are not included.