7C19
Crystal structure of a dinucleotide-binding protein (Y224A/Y246A) of ABC transporter endogenously bound to uridylyl-3'-5'-phospho-guanosine (Form II)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Sugar ABC transporter, periplasmic sugar-binding protein | polymer | 406 | 44320.6 | 2 | UniProt (Q5SLB4) Pfam (PF13416) In PDB | Thermus thermophilus HB8 | |
2 | A, B | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
3 | A, B | CARBON DIOXIDE | non-polymer | 44.0 | 2 | Chemie (CO2) | |||
4 | A | HYPOPHOSPHITE | non-polymer | 63.0 | 1 | Chemie (PO2) | |||
5 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
6 | A, B | [(1S,3R,3aR,6aS)-3-(2-azanyl-6-oxidanylidene-1H-purin-9-yl)-5,5-bis(oxidanyl)-1,3,3a,4,6,6a-hexahydrocyclopenta[c]furan-1-yl]methyl [(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-2-(hydroxymethyl)-4-oxidanyl-oxolan-3-yl] hydrogen phosphate | non-polymer | 629.5 | 2 | Chemie (FGO) | |||
7 | B | CARBONATE ION | non-polymer | 60.0 | 1 | Chemie (CO3) | |||
8 | B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
9 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
10 | water | water | 18.0 | 742 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 398 (UniProt: Q5SLB4)
PDB | External Database | Details |
---|---|---|
Met -1 | - | initiating methionine |
Met 0 | - | expression tag |
Ala 224 | Tyr 243 | engineered mutation |
Ala 246 | Tyr 265 | engineered mutation |
His 399 | - | expression tag |
His 400 | - | expression tag |
His 401 | - | expression tag |
His 402 | - | expression tag |
His 403 | - | expression tag |
His 404 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 88641.2 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 1801.1 | |
All* | Total formula weight | 90442.3 |