7C0J

Crystal structure of chimeric mutant of GH5 in complex with Z-DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Histone H5,Double-stranded RNA-specific adenosine deaminase	polymer	76	8109.3	2	UniProt (P02259) UniProt (P55265) Pfam (PF00538) Pfam (PF02295) In PDB	Gallus gallus (Chicken)
2	D, E	DNA (5'-D(*TP*CP*GP*CP*GP*CP*G)-3')	polymer	7	2114.4	2		synthetic construct
3		water	water		18.0	10	Chemie (HOH)

Sequence modifications

A, B: 25 - 64 (UniProt: P02259)

PDB	External Database	Details
Gly 20	-	expression tag
Ser 21	-	expression tag
His 22	-	expression tag
Met 23	-	expression tag
Ala 24	-	expression tag
Gly 41	Lys 41	engineered mutation
Glu 42	Ser 42	engineered mutation
Gly 43	Arg 43	engineered mutation
Ala 53	Lys 53	engineered mutation

A, B: 1169 - 1178 (UniProt: P55265)
A, B: 76 - 88 (UniProt: P02259)
A, B: 1192 - 1192 (UniProt: P55265)
A, B: 92 - 93 (UniProt: P02259)
A, B: 1195 - 1195 (UniProt: P55265)
A, B: 95 - 98 (UniProt: P02259)

PDB	External Database	Details
Ala 95	Arg 95	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	20447.3
All*	Total formula weight	20447.3

*Water molecules are not included.