7BY5

Tetanus neurotoxin mutant-(H233A/E234Q/H237A/Y375F)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Tetanus toxin	polymer	1322	151656.5	1	UniProt (P04958) Pfam (PF01742) Pfam (PF07952) Pfam (PF07953) Pfam (PF07951) In PDB	Clostridium tetani (strain Massachusetts / E88)	Tentoxylysin
2	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
3	A	1,2-ETHANEDIOL	non-polymer		62.1	8	Chemie (EDO)
4	A	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6		water	water		18.0	772	Chemie (HOH)

Sequence modifications

A: 2 - 1315 (UniProt: P04958)

PDB	External Database	Details
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Ala 233	His 233	engineered mutation
Gln 234	Glu 234	engineered mutation
Ala 237	His 237	engineered mutation
Phe 375	Tyr 375	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	151656.5
Non-Polymers*	Number of molecules	13
	Total formula weight	839.3
All*	Total formula weight	152495.8

*Water molecules are not included.