7BXT

The cryo-EM structure of CENP-A nucleosome in complex with CENP-C peptide and CENP-N N-terminal domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3,Histone H3-like centromeric protein A	polymer	144	16372.2	2	UniProt (Q6XXM1) Pfam (PF00125) UniProt (by SIFTS) (P84229)	Gallus gallus (Chicken)	Centromere protein A,GgCENP-A
2	B, F (B, F)	Histone H4	polymer	106	11676.7	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	133	14447.8	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (D, H)	Histone H2B type 2-E	polymer	129	14233.5	2	UniProt (Q16778) Pfam (PF00125)	Homo sapiens (Human)	H2B-clustered histone 21,Histone H2B-GL105,Histone H2B.q,H2B/q
5	I (I)	DNA (145-mer)	polymer	145	44529.4	1		synthetic construct
6	J (J)	DNA (145-mer)	polymer	145	44982.6	1		synthetic construct
7	K, L (K, L)	CENP-C	polymer	41	4932.8	2	UniProt (O57392)	Gallus gallus	Centromere protein CENP-C
8	M, N (M, N)	Maltodextrin-binding protein,Centromere protein N	polymer	630	71519.6	2	UniProt (Q1T765) Pfam (PF01547) Pfam (PF05238) UniProt (by SIFTS) (P0AEX9)	unidentified cloning vector	CENP-N

Sequence modifications

A, E: -2 - 63 (PDB: 7BXT)
A, E: 64 - 141 (UniProt: Q6XXM1)
B, F: 1 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

C, G: 1 - 129 (UniProt: P04908)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

D, H: 0 - 125 (UniProt: Q16778)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

M, N: -410 - 0 (PDB: 7BXT)
M, N: 1 - 211 (UniProt: Q1T765)

PDB	External Database	Details
Asp 212	-	expression tag
Pro 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	355877.5
All*	Total formula weight	355877.5

*Water molecules are not included.