7BVU

Crystal structure of S. thermophilus NFeoB E66A.E67A bound to GDP.AlF4-

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ferrous iron transport protein B	polymer	276	30498.6	2	UniProt (Q5M586) Pfam (PF02421) Pfam (PF17910)	Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311)
2	C, I (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
3	D, J (A, B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
4	E, K (A, B)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	2	Chemie (ALF)
5	F, L (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	G, M (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
7	H, N (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
8	O, P (A, B)	water	water		18.0	202	Chemie (HOH)

Sequence modifications

A, B: 1 - 270 (UniProt: Q5M586)

PDB	External Database	Details
Gly -5	-	expression tag
Ala -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 66	Glu 66	engineered mutation
Ala 67	Glu 67	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60997.1
Non-Polymers*	Number of molecules	12
	Total formula weight	1414.2
All*	Total formula weight	62411.3

*Water molecules are not included.