7BV1

Cryo-EM structure of the apo nsp12-nsp7-nsp8 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-directed RNA polymerase	polymer	951	109070.4	1	UniProt (P0DTD1) Pfam (PF06478) Pfam (PF00680)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,Pol,RdRp,Non-structural protein 12,nsp12
2	B, D (B, D)	Non-structural protein 8	polymer	207	23139.4	2	UniProt (P0DTD1) Pfam (PF08717)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,nsp8
3	C (C)	Non-structural protein 7	polymer	92	10485.2	1	UniProt (P0DTD1) Pfam (PF08716)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,nsp7
4	E, F (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 932 (UniProt: P0DTD1)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 933	-	expression tag
Gly 934	-	expression tag
Ser 935	-	expression tag
Glu 936	-	expression tag
Asn 937	-	expression tag
Leu 938	-	expression tag
Tyr 939	-	expression tag
Phe 940	-	expression tag
Gln 941	-	expression tag
Gly 942	-	expression tag
His 943	-	expression tag
His 944	-	expression tag
His 945	-	expression tag
His 946	-	expression tag
His 947	-	expression tag
His 948	-	expression tag
His 949	-	expression tag
His 950	-	expression tag

B, D: 1 - 198 (UniProt: P0DTD1)

PDB	External Database	Details
Met 0	-	initiating methionine
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag
His 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag
His 205	-	expression tag
His 206	-	expression tag

C: 1 - 83 (UniProt: P0DTD1)

PDB	External Database	Details
Met 0	-	initiating methionine
His 84	-	expression tag
His 85	-	expression tag
His 86	-	expression tag
His 87	-	expression tag
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
His 91	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	165834.5
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	165965.3

*Water molecules are not included.