7BRS
E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA02
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Beta-galactosidase | polymer | 1025 | 116688.5 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) In PDB | Escherichia coli K-12 | Beta-gal,Lactase |
2 | A, D, C, B | 8-[2-[(E)-2-[4-[(2S,3R,4S,5R,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxyphenyl]ethenyl]-3,3-dimethyl-indol-1-ium-1-yl]octanoic acid | non-polymer | 568.7 | 4 | Chemie (F4X) | |||
3 | B, A, D, C | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) | |||
4 | B, A, D, C | SODIUM ION | non-polymer | 23.0 | 7 | Chemie (NA) | |||
5 | C, B, A, D | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 46 | Chemie (DMS) | |||
6 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
7 | water | water | 18.0 | 382 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 0 - 1023 (UniProt: P00722)
PDB | External Database | Details |
---|---|---|
Ser -1 | - | expression tag |
Gln 537 | Glu 538 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 466754.2 | |
Non-Polymers* | Number of molecules | 66 |
Total formula weight | 6316.3 | |
All* | Total formula weight | 473070.5 |