7BO0

Crystal structure of ene-reductase GsOYE from Galdieria sulphuraria in complex with alpha-angelica lactone

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	NADPH2 dehydrogenase-like protein	polymer	397	45131.8	2	UniProt (M2XAQ9) Pfam (PF00724) In PDB	Galdieria sulphuraria (Red alga)
2	A	5-methyl-3~{H}-furan-2-one	non-polymer		98.1	1	Chemie (U6W)
3	A, B	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
4		water	water		18.0	448	Chemie (HOH)

Sequence modifications

A, B: 1 - 381 (UniProt: M2XAQ9)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 204	Thr 204	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	90263.6
Non-Polymers*	Number of molecules	3
	Total formula weight	1010.8
All*	Total formula weight	91274.4

*Water molecules are not included.