7BNQ

Lateral-closed conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Outer membrane protein assembly factor BamA	polymer	810	90601.4	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103)	Escherichia coli (strain K12)	Omp85
2	B (B)	Outer membrane protein assembly factor BamB	polymer	392	41918.9	1	UniProt (P77774) Pfam (PF01011) Pfam (PF13360)	Escherichia coli (strain K12)
3	C (C)	Outer membrane protein assembly factor BamC	polymer	344	36875.3	1	UniProt (P0A903) Pfam (PF06804)	Escherichia coli (strain K12)
4	D (D)	Outer membrane protein assembly factor BamD	polymer	245	27858.3	1	UniProt (P0AC02) Pfam (PF13525)	Escherichia coli (strain K12)
5	E (E)	Outer membrane protein assembly factor BamE	polymer	123	13530.3	1	UniProt (P0A937) Pfam (PF04355)	Escherichia coli (strain K12)

Sequence modifications

A: 1 - 810 (UniProt: P0A940)

PDB	External Database	Details
Cys 435	Glu 435	engineered mutation
Cys 665	Ser 665	engineered mutation
Ser 690	Cys 690	engineered mutation
Ser 700	Cys 700	engineered mutation

E: 1 - 113 (UniProt: P0A937)

PDB	External Database	Details
Gly 114	-	expression tag
Gly 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	210784.2
All*	Total formula weight	210784.2

*Water molecules are not included.