7BH1
Cryo-EM Structure of KdpFABC in E1 state with K
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Potassium-transporting ATPase potassium-binding subunit | polymer | 557 | 59247.7 | 1 | UniProt (P03959) Pfam (PF03814) In PDB | Escherichia coli K-12 | ATP phosphohydrolase [potassium-transporting] A chain,Potassium-binding and translocating subunit A,Potassium-translocating ATPase A chain |
2 | B | Potassium-transporting ATPase ATP-binding subunit | polymer | 682 | 72251.9 | 1 | UniProt (P03960) Pfam (PF00122) Pfam (PF00702) In PDB | Escherichia coli K-12 | ATP phosphohydrolase [potassium-transporting] B chain,Potassium-binding and translocating subunit B,Potassium-translocating ATPase B chain |
3 | C | Potassium-transporting ATPase KdpC subunit | polymer | 208 | 22299.2 | 1 | UniProt (P03961) Pfam (PF02669) In PDB | Escherichia coli K-12 | ATP phosphohydrolase [potassium-transporting] C chain,Potassium-binding and translocating subunit C,Potassium-translocating ATPase C chain |
4 | D | Potassium-transporting ATPase KdpF subunit | polymer | 29 | 3071.7 | 1 | UniProt (P36937) Pfam (PF09604) In PDB | Escherichia coli K-12 | ATP phosphohydrolase [potassium-transporting] F chain,Potassium-binding and translocating subunit F,Potassium-translocating ATPase F chain |
5 | A | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
6 | B | (2R)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (E)-octadec-9-enoate | non-polymer | 718.0 | 1 | Chemie (9Y0) | |||
7 | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A: 1 - 557 (UniProt: P03959)
B: 1 - 682 (UniProt: P03960)
C: 1 - 190 (UniProt: P03961)
PDB | External Database | Details |
---|---|---|
Arg 116 | Gln 116 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ala 162 | Ser 162 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 191 | - | expression tag |
Thr 192 | - | expression tag |
Gly 193 | - | expression tag |
Leu 194 | - | expression tag |
Val 195 | - | expression tag |
Pro 196 | - | expression tag |
Arg 197 | - | expression tag |
Gly 198 | - | expression tag |
Ser 199 | - | expression tag |
Ser 200 | - | expression tag |
His 201 | - | expression tag |
His 202 | - | expression tag |
His 203 | - | expression tag |
His 204 | - | expression tag |
His 205 | - | expression tag |
His 206 | - | expression tag |
His 207 | - | expression tag |
His 208 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 156870.5 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 757.1 | |
All* | Total formula weight | 157627.6 |