7BEO

Crystal structure of the receptor binding domain of SARS-CoV-2 Spike glycoprotein in a ternary complex with COVOX-253H55L and COVOX-75 Fabs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (R, X)	Spike glycoprotein	polymer	205	23150.9	2	UniProt (P0DTC2) Pfam (PF09408)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	S glycoprotein,E2,Peplomer protein
2	B, G (H, C)	COVOX-253H55L heavy chain	polymer	228	24371.4	2		Homo sapiens
3	C, H (L, D)	COVOX-253H55L light chain	polymer	215	23465.9	2		Homo sapiens
4	D, I (A, E)	COVOX-75 heavy chain	polymer	245	26133.4	2		Homo sapiens
5	E, J (B, F)	COVOX-75 light chain	polymer	227	24556.4	2		Homo sapiens
6	K, L, M (G, I, J)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	3	In PDB GlyTouCan (G21290RB)
7	N (R)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
8	O, T (H, X)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
9	P, Q, R, S, U... (L, A, C, D, E)	ACETATE ION	non-polymer		59.0	9	Chemie (ACT)
10	V (C)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)

Sequence modifications

R, X: 333 - 528 (UniProt: P0DTC2)

PDB	External Database	Details
Glu 324	-	expression tag
Thr 325	-	expression tag
Gly 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
Lys 527	Pro 527	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	243356.1
Branched	Number of molecules	3
	Total formula weight	1711.6
Non-Polymers*	Number of molecules	13
	Total formula weight	972.2
All*	Total formula weight	246040.0

*Water molecules are not included.