7BEM

Crystal structure of the receptor binding domain of SARS-CoV-2 Spike glycoprotein in complex with COVOX-269 scFv

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	H	COVOX-269 Vh domain	polymer	135	13950.3	1		Homo sapiens
2	L	COVOX-269 Vl domain	polymer	149	15553.2	1		Homo sapiens
3	E	Spike glycoprotein	polymer	205	23150.9	1	UniProt (P0DTC2) Pfam (PF09408) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	S glycoprotein,E2,Peplomer protein
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
5	H, E	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	H, E	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	L	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
8	E	PROLINE	non-polymer		115.1	4	Chemie (PRO)
9		water	water		18.0	33	Chemie (HOH)

Sequence modifications

E: 333 - 528 (UniProt: P0DTC2)

PDB	External Database	Details
Glu 324	-	expression tag
Thr 325	-	expression tag
Gly 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
Lys 527	Pro 527	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	52654.5
Branched	Number of molecules	1
	Total formula weight	570.5
Non-Polymers*	Number of molecules	10
	Total formula weight	905.6
All*	Total formula weight	54130.5

*Water molecules are not included.