7B6B

The carbohydrate binding module family 48 (CBM48) and carboxy-terminal carbohydrate esterase family 1 (CE1) domains of the multidomain esterase DmCE1B from Dysgonomonas mossii in complex with methyl ferulate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Carbohydrate Esterase family 1 protein with an N-terminal carbohydrate binding module family 48	polymer	386	43360.5	2	UniProt (F8X1N1) Pfam (PF00756) In PDB	Dysgonomonas mossii DSM 22836
2	A	Trans-methylferulate	non-polymer		208.2	2	Chemie (SZQ)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	A, B	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5		water	water		18.0	723	Chemie (HOH)

Sequence modifications

A, B: 292 - 656 (UniProt: F8X1N1)

PDB	External Database	Details
Met 271	-	initiating methionine
Gly 272	-	expression tag
Ser 273	-	expression tag
Ser 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
Ser 281	-	expression tag
Ser 282	-	expression tag
Glu 283	-	expression tag
Asn 284	-	expression tag
Leu 285	-	expression tag
Tyr 286	-	expression tag
Phe 287	-	expression tag
Gln 288	-	expression tag
Gly 289	-	expression tag
His 290	-	expression tag
Ser 291	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	86721.0
Non-Polymers*	Number of molecules	6
	Total formula weight	638.1
All*	Total formula weight	87359.1

*Water molecules are not included.