7B5O
Cryo-EM structure of the human CAK bound to ICEC0942 at 2.5 Angstroms resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (H) | CDK-activating kinase assembly factor MAT1 | polymer | 328 | 38132.3 | 1 | UniProt (P51948) Pfam (PF17121) Pfam (PF06391) | Homo sapiens (Human) | CDK7/cyclin-H assembly factor,Cyclin-G1-interacting protein,Menage a trois,RING finger protein 66,RING finger protein MAT1,p35,p36 |
| 2 | B (I) | Cyclin-H | polymer | 323 | 37695.5 | 1 | UniProt (P51946) Pfam (PF00134) Pfam (PF16899) | Homo sapiens (Human) | MO15-associated protein,p34,p37 |
| 3 | C (J) | Cyclin-dependent kinase 7 | polymer | 391 | 43651.1 | 1 | UniProt (P50613) Pfam (PF00069) | Homo sapiens (Human) | 39 kDa protein kinase,p39 Mo15,CDK-activating kinase 1,Cell division protein kinase 7,Serine/threonine-protein kinase 1,TFIIH basal transcription factor complex kinase subunit |
| 4 | D (J) | (3R,4R)-4-[[[7-[(phenylmethyl)amino]-3-propan-2-yl-pyrazolo[1,5-a]pyrimidin-5-yl]amino]methyl]piperidin-3-ol | non-polymer | 394.5 | 1 | Chemie (I74) | |||
| 5 | E, F, G (H, I, J) | water | water | 18.0 | 73 | Chemie (HOH) |
Sequence modifications
H: 1 - 309 (UniProt: P51948)
J: 1 - 346 (UniProt: P50613)
| PDB | External Database | Details |
|---|---|---|
| Met -18 | - | initiating methionine |
| Gly -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Ser -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -44 | - | initiating methionine |
| Ala -43 | - | expression tag |
| Ser -42 | - | expression tag |
| Trp -41 | - | expression tag |
| Ser -40 | - | expression tag |
| His -39 | - | expression tag |
| Pro -38 | - | expression tag |
| Gln -37 | - | expression tag |
| Phe -36 | - | expression tag |
| Glu -35 | - | expression tag |
| Lys -34 | - | expression tag |
| Gly -33 | - | expression tag |
| Gly -32 | - | expression tag |
| Gly -31 | - | expression tag |
| Ser -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Gly -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Ser -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Gly -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Ser -22 | - | expression tag |
| Trp -21 | - | expression tag |
| Ser -20 | - | expression tag |
| His -19 | - | expression tag |
| Pro -18 | - | expression tag |
| Gln -17 | - | expression tag |
| Phe -16 | - | expression tag |
| Glu -15 | - | expression tag |
| Lys -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 119478.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 394.5 | |
| All* | Total formula weight | 119873.4 |
