7B0R

In meso structure of the membrane integral lipoprotein intramolecular transacylase Lit from Bacillus cereus with H85R mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Hypothetical Membrane Spanning Protein	polymer	237	27659.8	1	UniProt (Q813T3) Pfam (PF07314)	Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
2	B, C, G (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	D, E, H, I (A)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	4	Chemie (OLC)
4	F (A)	3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL	non-polymer		398.5	1	Chemie (PE5)
5	J (A)	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
6	K (A)	1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE	non-polymer		178.2	1	Chemie (PG5)
7	L (A)	water	water		18.0	43	Chemie (HOH)

Sequence modifications

A: 1 - 218 (UniProt: Q813T3)

PDB	External Database	Details
Met -18	-	initiating methionine
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Arg -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Arg 85	His 85	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27659.8
Non-Polymers*	Number of molecules	10
	Total formula weight	2471.3
All*	Total formula weight	30131.1

*Water molecules are not included.