7B0H

TgoT_6G12 Ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	DNA (5'-D(P*AP*AP*CP*GP*GP*CP*AP*AP*AP*TP*GP*CP*G)-3')	polymer	13	4009.6	2		synthetic construct
2	C, F (D, G)	DNA (5'-D(P*CP*GP*CP*AP*TP*T)-3')	polymer	6	1784.2	2		synthetic construct
3	D, E (E, F)	DNA polymerase	polymer	773	89884.3	2	UniProt (P56689) Pfam (PF03104) Pfam (PF00136)	Thermococcus gorgonarius	TO POL
4	G, H, K, L (E, F)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
5	I, M (E, F)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	J, N (E, F)	THYMIDINE-5'-TRIPHOSPHATE	non-polymer		482.2	2	Chemie (TTP)

Sequence modifications

E, F: 1 - 773 (UniProt: P56689)

PDB	External Database	Details
Gln 93	Val 93	engineered mutation
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation
Leu 485	Ala 485	engineered mutation
Ala 589	Val 589	engineered mutation
Lys 609	Glu 609	engineered mutation
Met 610	Ile 610	engineered mutation
Gln 659	Lys 659	engineered mutation
Gln 664	Glu 664	engineered mutation
Pro 665	Gln 665	engineered mutation
Lys 668	Arg 668	engineered mutation
Gln 669	Asp 669	engineered mutation
His 671	Lys 671	engineered mutation
Arg 674	Lys 674	engineered mutation
Arg 676	Thr 676	engineered mutation
Ser 681	Ala 681	engineered mutation
Pro 704	Leu 704	engineered mutation
Gly 730	Glu 730	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	191356.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1232.7
All*	Total formula weight	192588.9

*Water molecules are not included.