7AZE

DNA polymerase sliding clamp from Escherichia coli with peptide 18 bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta sliding clamp	polymer	386	42801.9	2	UniProt (A0A073FMV0) Pfam (PF00712) Pfam (PF02767) Pfam (PF02768) UniProt (by SIFTS) (P0A988) In PDB	Escherichia coli 2-427-07_S4_C3
2	H, I	Peptide 18	polymer	6	822.9	2		synthetic construct
3	A	MALONATE ION	non-polymer		102.0	1	Chemie (MLI)
4	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	A, B	PENTAETHYLENE GLYCOL	non-polymer		238.3	3	Chemie (1PE)
6		water	water		18.0	740	Chemie (HOH)

Sequence modifications

A, B: 1 - 366 (UniProt: A0A073FMV0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	87249.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1001.1
All*	Total formula weight	88250.6

*Water molecules are not included.