7AZD

DNA polymerase sliding clamp from Escherichia coli with peptide 20 bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Beta sliding clamp	polymer	386	42801.9	4	UniProt (A0A073FMV0) Pfam (PF00712) Pfam (PF02767) Pfam (PF02768) UniProt (by SIFTS) (P0A988)	Escherichia coli 2-427-07_S4_C3
2	E, F, G, H (H, I, J, K)	Peptide 20	polymer	6	827.0	4		synthetic construct
3	I, L (A, B)	PENTAETHYLENE GLYCOL	non-polymer		238.3	2	Chemie (1PE)
4	J, K, M (A, B, C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
5	N, O, P, Q, R... (A, B, C, D, H...)	water	water		18.0	675	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 366 (UniProt: A0A073FMV0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	174515.4
Non-Polymers*	Number of molecules	5
	Total formula weight	794.9
All*	Total formula weight	175310.3

*Water molecules are not included.